Étude par chromatographie gazeuse de la variabilité des acides gras des graines de mil

Abstract

Gas chromatographic study of variations in the amounts of fatty acids in millet seeds To investigate new millet hybrids more accurate methods for the analysis of seed components are needed. For example, the study of small variations in amounts of fatty acids requires a better knowledge of sources of errors in their quantitative chromatographic determination. The results of the extraction—transmethylation method used have been verified by saponification and methylation. The resulting variability is not higher than that due to chromatographic determination (0.5%). Precautions to avoid some unexpected errors have been taken. The multivariate analysis of the results, seed by seed, makes it possible to collect those from the same ear. Sterols determined in these seeds cannot be used as biochemical tracers, since their quantities are too low and not sufficiently variable.