植物种子共轭亚麻酸含量分析_袁高峰（GC） . Analysis of conjugated linolenic acid from plant seeds

Abstract

The objective was to determine the lipid classes and conjugated linolenic acid(CLNA) contents in Trichosanthes kirilowii,pomegranate and bitter melon seed oils from China.Three pomegranate cultivars(Sanbaiyu,Qingpiruanzi and Tianlüzi) and six bitter melon cultivars(Changbai,Manyouqing,Bingchengyihao,Kaihua-1,Kaihua-2 and Qingfeng) were chosen for analysis.The predominant lipid was triacylglycerol in the analyzed samples ranging from 92.6% to 97.0%.The major fatty acids were oleic acid(22.6%,18:1),linoleic acid(32.6%,18:2n-6) and punicic acid(32.6%,c9,t11,c13-18:3) in T.kirilowii seed oil.The total CLNA was 36.9% in T.kirilowii seed oil.Punicic acid was the most predominant fatty acid,ranging from 73.4% to 77.5% and the total CLNA ranged from 83.4% to 87.9% in the seed oils of pomegranate cultivars.The total CLNA ranged from 65.2% to 71.9%,and α-eleostearic acid(c9,t11,t13-18:3) was predominant one,account from 56.0% to 63.7% in bitter melon seed oils.T.kirilowii,pomegranate and bitter melon were rich in CLNA,however,main CLNA in T.kirilowii and pomegranate was punicic acid,and one in bitter melon was α-eleostearic acid.Natural resources containing CLNA,especially edible T.kirilowii seed,could be a potential dietary source of CLA,following CLNA metabolism.