应用13C核磁共振研究完整野生大豆种子脂肪酸组成（13C核磁共振） . Study on Fatty-Acids Composition of Intact Wild Soybean Seed by Carbon-13 Nuclear Magnetic Resonance

Abstract

The fatty acid composition of some high-protein wild soybean and a series of wild, semiwild, semicultivated and cultivatad soybean intact seeds were determined by 13C NMR. All spectra were obtained with NMR spectrometer "operating at 25.05 MHz, completely proton decouping, a choice of 0.5 sec acquisition time and tip angle of around 45°. A given amount of intact seeds were immersed in D2O and all well resolved spectra achieved with accumulation for 3000 times (about 30 min.)The quantification of oil components was checked by the data of GC analysis and given a series of correlation coefficient.It had been found that (1) content of linolenic acid was lower in some high-protein (50%) wild soybean germplasm; (2) order of the linolenic acid content was: wild semiwild semi-cultivated cultivated soybean. 13C-NMR would be a powerful technique for study on genetics and breeding of soybean oil composition.