响应面法优化微波提取牡丹籽油的工艺研究_易军鹏 . Optimization of Microwave-assisted Extraction of Oil from Paeonia suffruticosa Andr. Seeds Using Response Surface Analysis

Abstract

Microwave was applied to the extraction of oil from Paeonia suffruticosa Andr. seeds. The extraction technique was optimized using response surface methodology. n-Hexane was selected as a optimal extraction solvent because compared to other solvents including petroleum ether (boiling range 30 - 60℃ or 60 - 90℃), ethyl acetate and acetone it yielded the most oil and can be easily penetrated by microwave. Effects of three crucial factors (i.e., liquid/material ratio, microwave power and extraction duration) on extraction yield of oil were investigated using one-factor-at-a-time design. Response surface central composition design in which the interactions between the variables were considered was applied to optimize these factors. A quadratic polynomial regression model was developed. The optimal microwave-assisted extraction conditions were as follows: liquid/material ratio (ml/g) 9:1, microwave power 825 W and extraction duration 8 min. Under such conditions, the actual oil yield was 24.52%, between which and the predicted value a relative error of about 0.01% was found. GC-MS Analysis indicated that the oil from Paeonia suffruticosa Andr. seeds was abundant in unsaturated fatty acids, in which the contents of linoleic acid and linolenic acid were 24.57% and 67.13%, respectively.