响应面法优化火麻仁油冷榨提取工艺_周鸿翔 . Optimization of Cold-Pressed Extraction Process for Hemp Seed Oil by Response Surface Methodology

Abstract

Objective:To obtain high quality,pure,natural hemp seed oil and meanwhile maintain the natural bio-activity of protein retained in hemp seed meal.Methods:One-factor-at-a-time design and response surface meth-odology were applied for the optimization of process parameters for the extraction of hemp seed oil by cold pressed method.A mathematical mode describing oil yield as a function of raw material water content,temperature,pressure and time was set up.The fatty acid composition and purity of hemp seed oil were analyzed by GC and peak area normalization method.Results:The optimal process parameters were determined through canonical analysis as follows:hemp seeds containing 4.5% water were pressed at 40 MPa and 59 ℃ for 36 min.Under these conditions,the yield of hemp seed oil was up to 82.74%.Hemp seed oil was found to be rich in linoleic acid,linolenic acid,oleic acid,arachidonic acid and other unsaturated fatty acids and have a total unsaturated fatty acids content as high as 89.80%.Conclusion:Response surface analysis is effective for optimizing the cold-pressed extraction of hemp seed oil.Hemp seed oil is rich in unsaturated fatty acids and can thus be seen as a functional oil with high nutritional value.