响应面法优化超声_微波协同萃取槟榔籽油及其脂肪酸组成分析_辛晓晨 . Optimization of Ultrasonic-Microwave Assisted Extraction of Betel Nut Oil by Response Surface Methodology and Analysis of Its Fatty Acid Composition

Abstract

Betel nut oil was obtained by ultrasonic-microwave assisted extraction and its compositions were rapidly determined by a spectrophotometer.The extraction process conditions were optimized by response surface tests on the basis of single factor tests.According to the effects of extraction time,extraction temperature and material-liquid ratio on extraction rate of betel nut oil,the optimal extraction process parameters were extraction time of 22 min,extraction temperature of 65 ℃ and materialliquid ratio of 1:11(g/mL).Under the optimal extraction process conditions,the extraction rate of betel nut oil was up to 14.29%.Gas chromatography analysis showed that the major compositions of betel nut oil were 33.5% myristic acid,20.4% 9-octadecenoic acid,19.19% 12-octadecadienoic acid,15.82% hexadecanoic acid,9.51% dodecanoic acid and 1.59% octadecanoic acid.