相应面试验化超临界CO2萃取胭脂萝卜籽油工艺及其脂肪酸组成分析（GC-MS) . Optimization of Supercritical CO2 Extraction of "Yanzhi" Red Radish Seed Oil and Analysis of Its Fatty Acid Composition

Abstract

Response surface methodology was used to optimize the extraction process of seed oil from “Yanzhi” red radish by supercritical CO2, and the fatty acid composition of the oil was determined. The Plackett-Burman design was used to evaluate the influence of seven factors on extraction efficiency, and three important extraction parameters, including extraction pressure, temperature and time, were selected. Steepest ascent path was adopted to approach the optimal response region of the three significant factors, and then response surface analysis based on Box-Behnken design was used to determine the optimal levels of the main factors. As a result, the optimal extraction conditions were established as follows: the extraction was conducted at 34 MPa and 44 ℃ for 91 min. Under these conditions, the predicted and experimental extraction rates of radish seed oil were 93.11% and (93.09 ± 0.80) %, respectively. The fatty acid composition of “Yanzhi” radish seed oil, as determined by gas chromatography tandem mass spectrometry (GC-MS), was similar to that of rapeseed oil. The contents of unsaturated and monounsaturated fatty acids in the radish seed oil were 89.01% and 67.50%, respectively.