水酶法提取火麻籽油的工艺优化及其脂肪酸组成分析_王欢李杨 . Optimization of Aqueous Enzymatic Extraction of Hemp Seed Oil and Analysis of Its Fatty Acid Composition

Abstract

The yields of hemp seed oil from peeled hemp seeds by aqueous enzymatic extraction with Protex 6L,Protex 7L,Alcalase 2.4L,Viscozyme L and Babaowei 2X were 3.62%,36.88%,17.05%,50.98%,and 33.33%,respectively.Viscozyme L was chosen as the best enzyme according to the oil contents in the emulsions,hydrolysates and precipitates.The enzymatic hydrolysis process was optimized by response surface methodology to obtain maximum free oil yield.As a result,the optimal hydrolysis conditions were determined as 7000 U/g of enzyme dosage,60 ℃,initial pH 5,1:6 of solid/liquid ratio and 3 h.By using GC analysis,11 fatty acids were detected in the seed oil,including saturated fatty acids(mainly palmitic,arachidic and stearic acid) and unsaturated fatty acids(mainly oleic,linoleic and α-linolenic acid).Among these fatty acids,the most abundant was linoleic acid with a relative content of 47.5%.