石榴籽油的超声辅助提取工艺及GC_MS分析_苗利利 . Ultrasonic wave auxiliary extraction technology for pomegranate seeds oil and analysis of fatty acid by GC-MS

Abstract

With pomegranate seeds as raw materials,the ultrasonic auxiliary extraction technology was used to extract its seed oil.The effects of particle size of material,solid/liquid ratio,extracting temperature and time,ultrasonic frequency were discussed by means of single experiments and orthogonal experiments,the optimum technical conditions were obtained and the combined application of gas chromatography-mass spectrometry(GC-MS) was employed to analyze the components of fatty acid of pomegranate seeds oil.The test results indicated that optimum conditions were as follows: solid/liquid ratio 1︰10(g/mL),ultrasonic frequency 60kHz,reacting temperature 40℃,time 60min,particle size of material 60 screen mesh,oil yield ratio could reach 29.0%.The main components of fatty acids of pomegranate seeds oil were: punicic acid 58.68%,eicosatrienoic acid 15.02%,oleic acid 6.46%,linoleic acid 6.06%,etc.