To study influence of temperature, pressure, time, co-solvent solution on extraction rate of recovery, fatty oil was extracted from Chicory seed by the supercritical CO2 extraction. The optimal approach has been identified with the extraction temperature 45℃, the pressure 35MPa, the isolated pressure I 7.0MPa, the isolated temperature I 45℃, the isolated pressure Ⅱ 6MPa, the isolated temperature Ⅱ 40℃ and extraction time 3 hours. Compared to the traditional solvent extraction approach, the time was shorter and result in avoiding degradation of unsaturated fatty acid. So, the better qualified product was obtained. According to GC-MS analysis, the main components of Chicory fatty acid is lauric acid, cardamom acid, plamitic acid, stearic acid, oleic acid, linoleic acid, arachidonic acid, γ-linolenic acid and sinapic acid. γ-linolenic acid takes 38% and 3.41% which shows significant difference in isolated I and isolated Ⅱ fatty acid respectively. It has been suggested that supercritical CO2 extraction approach is qualified for extraction, isolation, and collection of γ-linolenic acid.