牡丹籽油超临界CO_2萃取工艺优化及抗氧化活性的研究_史国安 . Optimization of Supercritical CO2 Extraction and Analysis of Antioxidation Activity of Peony Seed Oil

Abstract

Take peony seeds as raw material,supercritical CO2 extraction method was used to extract peony seed oil.To conduct single-factor experiment,three factors(temperature,pressure and time),which influence extraction rate were investigated.Take extraction rate as effect size,orthogonal experiment(L933) was designed with 3 main influence factors,that is temperature,pressure and time,to optimize the supercritical extraction process whose extraction condition is milder,and destroy to oxidation resistance component of oil is smaller.By DPPH method and the ferrous ions(Fe2+)-induced by peroxidation system,with oleic acid as control,the differences of the capacity of peony seed oil to scavenge DPPH free radicals and degree of peroxidation of two samples obtained by pressing and the supercritical CO2 extraction method respectively were investigated.The results showed that extraction time had the greatest impact on extraction rate,followed by extraction temperature,extraction pressure was the least.The optimal process conditions of supercritical CO2 extraction method were temperature 35 ℃,pressure 30 MPa and the time 60 min,the oil extraction rate was 28.86%.The content of unsaturated fatty acid reaches as high as 90% of peony seed oil,while the antioxidant property of seed oil is similar to fat-soluble antioxidant;Super critical oil's removal ability of DPPH radical was significantly higher than pressed oil,and lipid peroxide degree induced by Fe-2+ was less than pressed oil,which means supercritical peony seed oil extracted by supercritical CO2 extraction method superiors to pressed oil.So,supercritical CO2 extraction method is recommended for extracting high value-added peony seed oil.