酶法提取紫花苜蓿籽油的研究_许英一 . Enzymatic Extraction of Alfalfa Seed Oil

Abstract

The enzymatic method was employed to extract oil from alfalfa seeds.The ratio of compound enzymes and the optimal technique parameters of the oil extraction were determined by orthogonal tests.Results: The compound enzymes are formulated as AS1398 neutral protease dosage 0.4%,α-amylase dosage 0.5% and pectinase dosage 0.5%,material water ratio is 1:10,enzymolysis temperature is 50 ℃,and enzymolysis time is 6 h.The extraction rate reaches 11.23%.The extracted oil contains many kinds of fatty acid,linoleic acid 15.38%,oleic acid 14.45%,linonenic acid 11.20%,stearic acid 23.25%,and palmitic acid 10.24%.The physical and chemical properties of the alfalfa seed oil were studied.It is indicated that enzymatic extraction of oil from alfalfa seeds is feasible.