苦瓜籽中脂肪酸成分分析_吴丹 . GC determination of bitter melon seeds fatty acids

Abstract

The lipid were extracted by using methanol and chloroform after comminuting the seeds of bitter melon freezing at -80℃, and methylated by base-catalyzed methylation. Methylated lipids were purified by Thin Layer Chromatogram (TLC) and determined by GC equipped with 100m×0.25mm capillary column. The results indicated that the lipids were composed of 25 fatty acids among which the main component MUFA was 61.209% in which 24:1n9 was the highest one with 51.220%. The second highest oleic acid was 9.646%. The amount of SFA and PUFA was almost the same,19.228% and 19.563% respectively. The main PUFA was linoleic acid,13.416%. There were many kinds of CLA isomers that accounted for 3.870%. And 0.460% trans-18:1 were existed in bitter melon seeds which might be precursor of 9c,11t-CLA in human. In bitter melon seeds, CLA may be one of the functional components related to the function of anticancer, anti-atherogenesis, antibacterial, anti-virus or regulation of immunity.