苦瓜籽油脂肪酸成分的GC_MS分析_张飞 . GC-MS determination of bitter melon seeds oil fatty acids

Abstract

The bitter melon seeds oil was extracted by Soxhlet extraction method,and the physicochemical characteristics of bitter melon seed oil were determined including iodine value,saponification number,unsaponifiable matter,melting point.The bitter melon seed oil was esterified in the way of potassium hydroxide-methanol esterification at room temperature,and the fatty acids composition of bitter melon seeds oil was detected by GC-MS.The results show that,both of oil content(36.81%) and protein contein(43.05%) of bitter melon seed are higher,and the oil takes on white solid state,and sends out special,penetrating,bitter and astringent smell.The melting point,unsaponifiable matter,iodine value,saponification number of the bitter melon seeds oil are 53 ℃,0.9%,131 g/100 g and 196 mg/g,respectively.Eight kinds of fatty acids such as stearic acid(33.6%),α-eleostearic acid(42.3%),β-eleostearic acid(16.5%),palmitic acid(1.8%)、 oleic acid(2.3%)、 linoleic acid(2.9%)、 arachidic acid(0.4%)and eicosenoic(0.2%) are identified from bitter melon seeds oil.