绞股蓝种子油的提取、成分分析和急性毒性实验_刘世彪（GC-MS） . Extraction,composition analysis and acute toxicity test of seed oil of Gynostemma pentaphyllum

Abstract

Based on petroleum ether as extraction solvent and ratio of material to solution 1 ∶ 7,the ultrasonic-assisted extraction process on seed oil of Gynostemma pentaphyllum was optimized by single factor test and orthogonal test. The optimal ultrasonic-assisted conditions were as follows: extraction temperature 60 ℃,ultrasonic power 200 W and extraction time 50 min,under which the extraction rate of seed oil could reach 28. 13%. Physicochemical properties of the seed oil including density,dioptre,acid value,saponification value and iodine value were respectively 0. 895 g·mL-1,1. 518 n D 20,0.85,180.2 mg KOH·g-1and 135. 7 g I 2 ·100g-1,which revealed that G. pentaphyllum seed oil was a fine drying oil. GC-MS analysis revealed that there were 10 kinds of fatty acids in G. pentaphyllum seed oil,and the mass fraction were linolenic acid( 80. 49%),oleic acid( 8. 05%),linoleic acid( 7. 08%),stearic acid( 1. 79%) and palmitic acid( 1.28%). The unsaturated fatty acids accounted for 96.48% of total fatty acids. In the acute toxicity test,a dose of 65 mL·( kg · d)-1of seed oil were filled into mice's stomach,and no evident poisoning symptoms and animal death were detected after 14 d continuous observation,which preliminarily indicated that G. pentaphyllum seed oil was of nontoxicity,and expected to be developed as a resource of health oil.