火麻仁油中脂肪酸的不同酯化方法与GC_MS分析_李文峰 . Analysis of fatty acids in hemp seed oil using three different esterification methods by GC-MS

Abstract

The hemp seed oil was extracted by Soxhletex traction.The fatty acids in the hemp seed oil were esterified by three methods and analyzed by gas chromatography-mass spectrometry(GC-MS).The components and their relative contents of the fatty acids were compared with each other.The results showed that 14 fatty acids were identified by esterification method 1,11 fatty acids were identified by esterification method 2 and 10 fatty acids were identified by esterification method 3.The contents of the identified fatty acids accounted for 83.36%,99.63% and 96.98% of the hemp seed oil,respectively.Comparison of the major fatty acids was made:the major fatty acids were hexadecanoic acid(10.97%),octadecanoic acid(7.56%),9,12-octadecadienoic acid(13.42%)and 9-octadecenoicacid(34.80%)by esterification method 1.The major fatty acids were hexadecanoic acid(8.33%),octadecanoic acid(5.53%),9,12-octadecadienoic acid(54.64%)and 9,12,15-octadecatrienoic acid(25.42%)by esterification method 2.The major fatty acids were hexadecanoic acid(8.20%),octadecanoic acid(5.23%),9,12-octadecadienoic acid(52.19%)and 9,12,15-octadecatrienoic acid(25.77%)by esterification method 3.