花生种子脂肪酸含量的微量快速测定_高慧敏 . Microanalysis and Fast Analysis on Fatty Acids of Peanut Seed by Gas Chromatography

Abstract

The content of fatty acids is an important index for oil crops.The traditional methods of detectingfatty acids need more samples and more time,which is difficult to select hundreds of materials for breeders.Todetect the fatty acids or to propagate is ambivalent when the material detected has few seeds.In thisexperiment,a fast microanalysis was studied for detecting fatty acids of peanut.A part of about 0.020 g cut fromone seed of peanut was used,extracted oil using the mixture(benzene:petroleum ether=1:1) for 5 minutes,andmethyl esterificated using sodium hydroxide-methanol solution(c = 0.5 mol/L) for 10 minutes,then thesupernatant fluid was injected into the GC system.Using this method,the work which usually need severalhours,now can be completed in 15 min,thus lots of time can be saved.By comparing this method withtraditional method(GB/T 5009.6-2003,GB/T 17376-1998) for detecting fatty acid compositions,it wasconfirmed that the results got from this method was exact and credible.