番茄少量种子脂肪酸组成含量分析方法研究_秦公伟 . Research on Analysis Method of Fatty Acid Composition in Small Tomato Seeds

Abstract

The composition of fatty acids in rude fat which was extracted from small tomato seeds and modified to methyl esters were detected by Gas chromatography(GC),using supelco 37 fatty acid methyl ester Mixture(37 FAME) as control.The result showed that a fast analysis method was established for detecting relative percentage content of fatty acids in a small tomato seeds.Rude fat was extracted from a 4mg sample using isooctane for 5 minutes,and it was esterified using potassium hydroxide-methanol solution(2 mol / L) for 5minutes,then the solution was injected into the GC system.Using this method,the work which usually need several hours can be completed in 20 mins now,thus lots of time can be saved.The value of fatty acid composition in tomato seeds are not affected by Sample Weight,crude fat extraction time and fatty acid esterification time.Comparing this method with traditional method(GB / T 5009.6-2003,GB / T 17376-2008 5 ester exchange method) for detecting fatty acids composition,it was confirmed that the results got from this method was accurate and feasible.