杜仲籽油混合脂肪酸制备工艺优化及脂肪酸组成分析_吴丽雅 . Optimization of the preparation process of eucommia seed oil mixed fatty acid and the analysis of its fatty acid composition

Abstract

With the eucommia ulmoids oliv seed oil as raw material,eucommia ulmoids oliv seed oil mxed fatty acids was prepared by saponification,acid hydrolysis and extraction by n-hexane under nomal pressure.According to the single factor experiments,super alkaline,saponification temperature,saponification time and acidification pH were selected.The Box-behnken design was used to optimize of saponification and to set up a regression model,the fatty acid composition of fatty acid was analyzed by GC-MS.Results showed that the optimum condition were excess alkali 52%,saponification temperature 77℃,saponification time 82min,acid hydrolysis pH value 2.7,leading to fatty acid value of 159.19mg KOH/g.The relative content of alpha linolenic acid reaches 60.14% by analysing of GC-MS.It could be used as a further separation and extraction of eucommia seed oil of alpha linolenic acid raw material.