不同南瓜子脂肪酸成分的GC分析_唐丽君 . GC Determination of Fatty Acids in Different Pumpkin Seeds

Abstract

Lipids were extracted by using methanol and chloroform after comminuting the seeds of traditional pumpkin,pumkin squash and tea pumpkin of qiaqia’s in the environment of dry ice. Different components of fatty acids were methylated. Methylated lipids were then separated by Thin layer chromatogram (TLC) and determined by GC equipped with 100m×0.25mm capillary column. The results indicated that kinds of the seeds and whether they were processed had little influence on the content of fatty acid. The lipids composed of 17 fatty acids,among which monounsaturated fatty acids (MUFA)was 55.77%,polyunsaturated fatty acids (PUFA)was 30.26% and saturated fatty acids (SPA) was 28.46%. The main PUFA was linoleic acid,which was 29.71%.