不同方式提取的柑橘种子油中脂肪酸组成及活性成分分析GC-MS . Analysis of Fatty Acid Composition and Active Substance Content of Citrus Seed Oils Extracted by Different Methods

Abstract

The purpose of this study was to explore the impact of extraction method and citrus variety on the fatty acid composition and active substance content of citrus seed oil. The seeds of tangerine(Citrus reticulate), long-leafed sweet orange(Citrus sinensis L. Osbeck) and Liangping pomelo(Citrus maxima cv. Liangping Yu) were used in this study. A total of 12 citrus seed oils were extracted from the seed samples by supercritical CO_2 fluid extraction, ultrasonic-assisted solvent extraction, mechanical cold pressing, and hot pressing, respectively. The citrus seed oils contained 5 main fatty acids, palmitic acid, stearic acid, palmitic acid, oleic acid, and linoleic acid as measured by GC-MS. Some samples also contained functional fatty acids such as dihomo-γ-linolenic acid and squalene. The extraction methods had a significant effect on fatty acid composition of oils. The contents of limonoids, nomilin, and β-sitosterol in citrus seed oils were 0.05-0.99 mg/g, 0.13-0.98 mg/g, and 20.25-55.04 μg/g, respectively as determined by HPLC. The extraction methods had a significant effect on the β-sitosterol content, and an extremely significant effect on the limonoids content.