9种野生牡丹籽油主要脂肪酸成分分析 GC-MS . Analysis of fatty acid in seed oil from nine wild peony species

Abstract

Analyzing and evaluating fatty acid compositions of peony seed oil from different peony species are significant for selecting new varieties and oil peony breeding. Supercritical CO2 Extraction has been applied to extract peony seed oil from nine wild species of peonies and one cultivar peony (as control group). The fatty acid compositions have been determined by gas chromatography-mass spectrometry (GC-MS) and quantified by internal standard method. The experimental data have been analyzed through one-way ANOVA. The results indicated that the oil contents were different among different peony species, and oil extraction rate of Paeonia ostii was the highest (22.31%). Peony seed oil was rich in unsaturated fatty acids, which included linolenic acid, linoleic acid and oleic acid. There were significant differences in the total contents of fatty acids among different species P.potaninii showed the highest content of total fatty acids (90.29 g/100 g crude oil) which were significantly higher than that of the control P. ostii`Feng Dan'. There were also obvious differences in the contents of monomer fatty acid among different species. Linolenic acid content of P. potaninii (39.45 g/100 g crude oil)was almost three times of that of P. decomposita, and linoleic acid content of P. ostii 'Feng Dan' (25.06 g/100 g crude oil) was also distinctly higher than that of P.qiui (9.03 g/100 g crude oil). In conclusion, for the selection of new varieties and germplasm resources evaluation of tree peony, the differences among species and ecotypes should be taken into consideration.