Michigan State University
Oil in Biota orientalis seed was extracted using n-hexane with the assistance of ultrasound. Effects of liquid-to-material ratio, extraction time, extraction temperature and ultrasound power on the yield of oil were investigated by conducting single factor tests. Subsequently, a central composite design was employed and the results were analyzed by using response surface methodology. The optimal extraction conditions were as follows: liquid-to-material ratio 7:1 , extraction time 38 min, extraction temperature 55 ℃,ultrasound power 270 W. Under such conditions, the yield of the oil was 93.47%. Gas chromatography-mass spectrometry analysis indicated that the oil was abundant in unsaturated fatty acids, accounting for 84.37% of the total fatty acids and consisting of 28.4.1% of oleic acid , 11.40% of linoleic acid and 44.56% of linolenic acid.