Analysis of triglycerides by consecutive chromatographic techniques. i. cuphea llavia seed fat

Abstract

The triglycerides of Cuphea llavia var. miniata seed fat were separated according to the number of double bonds/molecule using preparative thin layer chromatography (TLC) on silicic acid impregnated with silver ion. The recovered frac-tions were quantitated by the chromotropic acid technique. Each fraction was then separated ac-cording to mol wt using gas-liquid chromatog-raphy (GLC). This multiple chromatography procedure resolved C. llavia triglycerides into 17 different components. The triglyceride composition of C. llavia seed fat was calculated from the above results. Since the fat contains 91.2 mole % decanoic acid, it was expected that each triglyceride molecule would contain at least two molecules of decanoic acid. Results showed this to be generally true, but sev-eral minor component triglycerides not conform-ing to this pattern were found. © 1964 American Oil Chemists' Society.