Exploring Phylogenetic Relationships between Hundreds of Plant Fatty Acids Synthesized by Thousands of Plants.
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Seed fats of eight species of Lauraceae (laurel family), six species of Cuphea (Lythraceae family), and three species of Ulmaceae (elm family) were extracted, and the triglycerides were isolated by preparative thin-layer chromatography. GLC of the triglycerides on a silicone column resolved 10 to 18 peaks with a 22 to 58 carbon number range for each fat. These carbon number distributions yielded considerable information about triglyceride compositions of the fats. The most interesting finding was with Laurus nobilis seed fat, which contained 58.4% lauric acid and 29.2-29.8% trilaurin. A maximum of 19.9% trilaurin would be predicted by a 1, 2, 3-random, a 1, 3-random-2-random, or a 1-random-2-random-3-random distribution of the lauric acid 3. This indicates a specificity for the biosynthesis of a simple triglyceride by Laurus nobilis seed enzymes. Cuphea lanceolata seed fat also contained more simple triglyceride (tridecanoin) than would be predicted by the fatty acid distribution theories. © 1967 American Oil Chemists' Society.
Authors: Litchfield, C.; Miller, E.; Harlow, R. D.; Reiser, R.
Journal: Lipids
Year: 1967
Volume: 2
Page: 345-350
UID: WOS:A19679682900012
DOI: 10.1007/BF02532124