STEREOSPECIFIC ANALYSIS OF TRIACYLGLYCERIDES WITH RARELY OCCURRING FATTY-ACIDS

Abstract

The glyceride structure of seed oils which contain each a rarely occurring fatty acid at a higher amount, is investigated. Seeds of 12 plants from three families are available. Besides the stereospecific analysis of the triglycerides by lipases of pancreas and of various mushrooms partial deacylation with ethylmagnesium bromide are carried out. By means of reverse phase HPLC a separation in individual triacylglycerides is performed; even a separation of such glycerides takes place which only differ in one isomeric fatty acid. The distribution of the fatty acids on the sn-positions of glycerol does not follow in all plants the hypothesis of C. D. Evans.