Exploring Phylogenetic Relationships between Hundreds of Plant Fatty Acids Synthesized by Thousands of Plants. more details ...
Abstract The triacylglycerols (TAGs) of the pulp of safou (Dacryodes edulis) were first fractionated by high-performance liquid chromatography (HPLC) using a column packed with silica impregnated with silver ions (Ag+) into 13 classes of increasing unsaturation. The 5 major classes of TAGs were again fractionated by reversed-phase HPLC according to carbon number. Each class contained one major TAG (more than 95% of the class TAGs) which was collected and submitted to partial deacylation by porcine pancreatic lipase. The 2-acyl-sn-glycerols (sn2-MAGs) formed were isolated by thin-layer chromatography and their constituent fatty acids were analyzed by gas-liquid chromatography. The proportion of 11 TAGs whose fatty acid at the sn2-position was known were determined. These 11 TAGs together amounted to more than 90% of the total TAGs. The major (> 15%) TAGs were : 2-oleoyl-1(3)-dipalmitoyloleoyl-sn-glycerol (16:0-18:1-16:0 = 27.1%), 2-oleoyl-1(3)-palmitoytoleoyl-sn-glycerol (16:0-18:1-18:1 = 21.7%) and 2-linoleoyl-1(3)-dipalmitoyloleoyl-sn-glycerol (16:0-182-16:0 = 16.2%). Twenty one other minor TAGs, whose fatty acid positional distribution was unknown, were also identified and quantified. They only represented 7.5% of the total.
Citation

Authors: Kinkela, T.; Bezard, J.

Journal: Revue Francaise Des Corps Gras

Year: 1993

Volume: 40

Page: 373-382

UID: WOS:A1993NA78000003

Plants
Genus Species Data Points