Triglyceride Composition of Walnut Oil

Abstract

"The present study was conducted to elucidate the triglyceride composition of walnut oil. The trigly cerides were separated by thin layer chromatography (TLC) and fractionated on the basis of partition numbers by reverse phase high performance liquid chromatography (HPLC) on a column packed with t<-bondapak C,8 using methanol-chloroform mixture as a solvent system. Each of these collected fractions was fractionated again on the basis of acyl carbon number of triglyceride by gas liquid chromatography (GLC). The fatty acid composition of triglycerides for each partition numbered group was also analyzed by GLC. From the results, it was found that walnut oil consists of ten kinds of triglycerides, and the patterns of major ones in walnut oil were as follows: 53. SJG of (Crs:2, Crs:2, Crs:2), 10.1% of (Cts:h, C1s:2, CIB:z), 5.4% of (Cts:h, C18:1, Cts:2), 4. 3% of (C1s:I, Cts:2, C1s:s), 3. 9% of (Ct6:0, Cts:2, Cts:2), 2.0% of (Ct6:o, C1s:t, C1a:2), 1.8% of (ClS:o, C1a:2, Cta:2). "